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KMID : 0613820020120020158
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Journal of Life Science
2002 Volume.12 No. 2 p.158 ~ p.163
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Axenic Isolation and 16S rRNA Gene Sequence of the Cyanobacterium Microcystis aeruginosa in Downstream of Nakdong River
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Park Hong-Gi
Jung Eun-Young
Lee You-Jung
Jung Jong-Moon
Hong Yong-Ki
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Abstract
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For axenic isolation of the cyanobacterium Microcystis aeruginosa, water bloom at the Mulgum station from the Nakdong River was pretreated by shaking with distilled water. Removal of bacteria was accomplished using antibiotics (150 ㎍/㎖ ampicillin and 25 ㎍/㎖ neomycin) and colonizing on CB solid medium prepared from 0.7% agarose at 30℃ under 40 μmol m-² s-¹ light. Among 26 strains of the Microcystis species, only three strains were axenically established. The three strains were examined by PCR-amplified 16S rRNA gene and 16S rRNA sequencing. The similarities were 99.5 ～ 100% with M. aeruginosa AF 139292.
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KEYWORD
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Agarose, Antibiotics, Microcystis aeruginosa, PCR, 16S rRNA primer
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